Surgery Promotes Implantation of Disseminated Tumor Cells, but Does Not Increase Growth of Tumor Cell Clusters


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Abstract

Introduction:Local recurrence and peritoneal dissemination is common after intentionally curative resection of colorectal carcinoma. It is not yet clear which mechanisms stimulate post-operative intra-abdominal tumor development. Enhanced adhesion or growth of tumor cells and/or post-operative immuno suppression may influence tumor recurrence.Aims of the study:In the present study, we evaluated effects of local and remote surgery on intra-abdominal tumor development.Materials and Methods:A standardized intra-abdominal trauma was inflicted by rubbing both uterus horns in laparotomy groups, while a dorsolateral thoracotomy was performed in thoracotomy groups (on day -1, 0, or +3). To induce tumor development rats were injected intra-peritoneally with the coloncarcinoma cell line CC531s on day 0 and evaluated after 21 days.Results:Rats undergoing laparotomy and injection on day 0 showed significantly higher tumorload than control rats (195 ± 20 vs. 47 ± 29, P < 0.001). When a laparotomy was performed, the day before tumor inoculation even higher tumorload was seen (245 ± 37 vs. 195 ± 20, P < 0.01). Strikingly, performing a thoracotomy on the day before or on the same day as tumor inoculation resulted in enhanced tumorload compared to controls as well (135 ± 84 vs. 47 ± 29; P < 0.001 and 88 ± 38 vs. 47 ± 29; P < 0.02, respectively). Either laparotomy or thoracotomy 3 days after tumor cell inoculation did not affect growth of pre-existing tumor cell clusters.Conclusions:The (post) surgical intra-peritoneal microenvironment enhances successful implantation of spilled tumor cells, whereas growth of adhered tumor cell clusters is not affected. The inflammatory response as a result of remote surgery promotes successful tumor development as well.

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