Incidence of eye leaf spot disease caused byHelminthosporium tetramerain different sugarcane varieties was recorded in Tarai belt of Uttar Pradesh during 1998-99 and 1999-2000 crop seasons in plant as well as ratoon crop. The disease incidence was more severe in plants (27.65%, 32.14%) followed by ratoons (28.40%, 44.46%) of cultivar CoS 767 in both crop seasons, respectively followed by CoS 91269 and CoS 90265. Essential oil ofCitrus sinensisand aquatic extract of garlic bulb at 2000 ppm were found to inhibit completely the mycelial growth and sporulation ofH. tetramera.However, onion bulb and eucalyptus leaf extract at 3000 ppm had also significant inhibitory effect on the fungus. Among the six fungicides testedin vitroagainst the pathogen, bayleton was found most effective in inhibiting the mycelial growth and sporulation. These fungicides were also tested under field condition for their control efficacy against the fungus and found that blitox-50 was more effective to control (71.37%) the eye spot disease of sugarcane followed by bayleton (59.26%) and carbendazim(53.64%).