Comparative study on the effect of live bees’ stings versus their venom on a model of hepatic fibrosis in albino rats: a histological and immunohistochemical study

    loading  Checking for direct PDF access through Ovid



Bee venom has a long tradition of use in the control of pain and inflammation in various chronic diseases. It is found to have antifibrotic activity especially on hepatocytes. Chronic liver diseases are a major cause of mortality and morbidity worldwide and represent a major health problem in Egypt.


The aim of the study was to compare between the effects of live bee stings and extracted bee venom on a model of liver fibrosis induced by common bile duct ligation in rats.

Materials and methods

Thirty-five adult male albino rats were divided randomly into four groups. Group I was the sham group. Group II was the common bile duct ligation group. Group III was the common bile duct ligation followed by direct bee sting group. Group IV was the common bile duct ligation followed by extracted bee venom group. Liver function was assessed. Histological study of the experimental groups was carried out using hematoxylin and eosin and sirius red stains. Immunohistochemical study for BCL2 was carried out, followed by morphometric and statistical studies. Electron microscopic study was also conducted.


Common bile duct ligation induced hepatocellular degeneration, cellular infiltration, and bile duct proliferation. Treatment with bee venom after common bile duct ligation resulted in marked improvement in liver enzymes, histological findings, and immunohistochemical expression of BCL2. In contrast, treatment with bee sting resulted in partial improvement.


In conclusion, bee venom is an important therapeutic agent in hepatic fibrosis because of its antifibrotic, anti-inflammatory, and antiapoptotic properties. However, extracted bee venom is better recommended than bee sting because of allergic manifestations and exaggerated inflammatory responses of the latter (sting).

Related Topics

    loading  Loading Related Articles