Poly(lactic-co-glycolic acid) (PLGA) nanoparticles containing both paclitaxel (PTX) and gemcitabine hydrochloride (GEM) were prepared and the effect of lyophilization and spray drying on physicochemical characteristics of these nanoparticles were evaluated.Methods:
Nanoemulsions were prepared by oil-in-water emulsion solvent diffusion technique using sonication and high-pressure homogenization. Nanoemulsion was dried using lyophilization or spray drying and drug content analyzed by high-performance liquid chromatography (HPLC).Results:
The particle size of the nanoemulsion was 183.6 ± 10.8 nm and the entrapment efficiency for PTX and GEM was 72.01 ± 1.35% and 6.95 ± 3.97%. Many properties including particle size, stability, surface morphology, moisture content, release characteristic and cellular uptake in MDCK and MDA-MB-231 cells were affected by the method of drying.Conclusion:
The lyophilized nanoparticles were smaller in size with higher stability and cellular uptake than spray-dried nanoparticles.