MicroRNAs 143 and 145 may be involved in benign prostatic hyperplasia pathogenesis through regulation of target genes and proteins

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Abstract

Objectives:

The aim of this study was to analyze the roles of miR-143 and miR-145, as well as the gene and protein expression of their targets (KRAS,ERK5,MAP3K3, andMAP4K4) in the pathogenesis of benign prostatic hyperplasia (BPH).

Methods:

We analyzed the specimens of 44 patients diagnosed with BPH who underwent surgical treatment. The control group consisted of prostate samples from 2 young patients who were organ donors. miRNAs and their target genes were assessed using real-time polymerase chain reaction (qRT-PCR), and protein levels were assessed by Western blotting.

Results:

miR-143 and miR-145 were overexpressed in, respectively, 62.5% and 73.8% of the cases. TheERK5andMAP4K4genes were underexpressed respectively in 59.4% and 100% of the BPH samples, whereasKRASandMAP3K3were overexpressed respectively in 79.4% and 61.5% of the samples. Increased protein expression was found for both KRAS (4,312.2 luminance/area) and MAP3K3 (7,461.7 luminance/area), while the ERK5 protein was more abundant in the samples from patients with prostate larger than 60 grams (p=0.019).

Conclusions:

The overexpression of miR-143 and miR-145 in BPH samples suggests an association with the pathogenesis of the disease; additionally, the latter miRNA may act through the inhibition of MAP4K4.KRASandMAP3K3overexpression may also be associated with BPH pathogenesis. Further analyses are necessary to confirm these results.

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