Research examining how personality is related to bullying has focused mainly on the personality of the target. The aim of this study was to examine instigators of workplace bullying to determine whether there was a relationship between the Big Five personality factors and the incidence of bullying. The sample consisted of 129 individuals who completed an online survey via Amazon Mechanical Turk. Results indicated that conscientiousness and agreeableness had a negative relationship with bullying, whereas neuroticism had a positive relationship with bullying. These results provide support for using personality assessments as part of a screening procedure for organizations so that workplace bullying can be effectively eradicated, or at least severely limited.