In orthotopic liver transplantation in the rat, cuff techniques have been developed for anastomoses of the portal vein and bile duct. These techniques have shortened the clamping time of the portal vein and have resulted in fewer biliary complications improving survival of the liver graft. Eighty-three per cent of the grafted animals have survived for 1 week and several animals have survived for more than 6 months.
We believe that use of the cuff method technically simplifies microvascular anastomoses. It may be particularly useful for anastomosis of the portal vein after machine preservation of the donor rat liver.