EFFECT OF TEMPERATURE ON HEPATIC AND RENAL UPTAKE OF COMPONENTS FROM UNIVERSITY OF WISCONSIN SOLUTION

    loading  Checking for direct PDF access through Ovid

Abstract

In rat liver and kidney preservation, hepatic and renal uptake of3 H-adenosine, 3H-glutathione, and 3H-raffinose from University of Wisconsin solution and diffusion to the interstitial space were measured. At 4°C only 0.38±0.47% and 2±0.92% of the total3 H-adenosine remained in the kidney and in the liver, respectively, but at 37°C the amount remaining was 1±1% and 12±3%(P<0.001). Hepatic and renal uptake of the impermeant3 H-raffinose was unaffected by temperature. During flush out, interstitial accumulation of adenosine was significantly higher in livers than in kidneys and decreased during 24-h cold storage. Glutathione accumulation in the interstitial space was two orders of magnitude lower than3 H-adenosine accumulation and comparable to the impermeant raffinose. In summary, the bioavailability of components of preservation solutions at 4°C is lower than at physiological temperatures, so that the application of cytoprotectants at 37°C to organ donors, rather than simple addition to the cold storage solution, might improve cold storage preservation of livers and kidneys.

Related Topics

    loading  Loading Related Articles