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Organ donation after citizens’ death was initiated in the 2010s and great progress had been made since then. But the willingness of organ donation still very low. Our research aimed to investigate the knowledge, attitudes and willingness of organ donation in medical students and identified the factors attributing to organ donation.A simple randomized sampling method was adopted. Medical students who were selected from two medical universities were investigated from November 2015 to January 2016 in Tianjin, China. Numerical data were summed up as means and standard deviations. Enumeration data were summarized as frequencies and percentages. The difference between two groups was examined by chi square test or Fisher exact test and a p value less than 0.05 was considered statistically significant.400 questionnaires were distributed and withdrew immediately, and 341 (341/400, 85.25%) questionnaires were valid, and used for analysis. Our research revealed that 85.3% respondents were willing to donate. Participants who had blood donation experience showed more positive willingness of organ donation (=5.42, p = 0.02). We found that the positive group was easier to accept the question of “whether you would donate your organs when you applied for a driver license” (=12.13, p = 0.002). The majority participants knew liver (90.6%), kidney (94.1%) and cornea (91.2%) can be transplanted, however, only a few students knew pancreas (13.2%) and intestine (14.4%) can be transplanted (=4.04, p = 0.543). Newspaper and books (71.8%), internet (55.7%) and public service announcements (39.3%) were the most important ways to achieve information for participants, however, only few students (4.5%) implied that they had ever got a course of lecture at school. Those who held negative willingness expressed that traditional values (72.0%), disfigured corpse (66.0%) and imperfect legal system (54.0%) were main reasons.Medical students showed more positive willingness than other people in China, but the rate was still less than medical students in other countries. The comprehensive publicity policy was defective, and campus donation-related education was lacking. Confucian culture had a greater impact on the participants. Medical students as medical successors should accept more organ donation education, so as to let the people around them to recognize the importance of organ donation.References:1. Sui W, Zheng C. Yang M, et al. organ donation in china: current status, challenges, and future development. Prog transplant 2015; 24:375-80.2. H. Zhang, J. Zheng,W.Liu, et al. Investigation and Strategic Analysis of Public Willingness and Attitudes Toward Organ Donation in East China. Transplantation Proc. 2015; 47:2419–2424.3. J. Chung, D, Choi, and Y, Park. Knowledge and Opinion of Deceased Organ Donation among Middle and High School Students in Korea.Transplantation Proc. 2015; 47:2805–09.4. Zhang GY, Liao T, and Fu XB, et al. Organ transplantation in China: concerns remain. Lancet 2014; 383:854–5.5. Weaning China off organs from executed prisoners. Lancet 2015; 385:16. Dai S. Xu L. China to stop using organs from executed prisoners for transplantations. BMJ 2015; 350:h239.7. Tokalak I, Kut A, Moray G, et al. Knowledge and attitudes of high school students related to organ donation and transplantation: a cross-sectional survey in Turkey. Saudi J Kidney Dis Transpl.2006; 17:491–496.8. Sander SL, Miller BK. Public knowledge and attitudes regarding organ and tissue donation: an analysis of the northwest Ohio community. Patient EducCouns.2005; 58:154–163.9. Figueroa CA, Mesfum ET, Acton NT, et al. Medical students’ knowledge and attitudes toward organ donation: results of a Dutch survey. Transplant Proc. 2013; 45:2093–2097.10. Boey KW. A cross-validation study of nurses’ attitudes and commitment to organ donation in Hong Kong.Int J Nurs Stud. 2002;39:95–104.11. Salim A, Malinoski D, Schulman D, Desai C, Navarro S, Ley EJ. The combination of an online organ and tissue registry with a public education campaign can increase the number of organs available for transplantation. J Trauma 2010; 69:451-4.12. Trornton JD, Wong KA, Cardenas V, Curtis JR, Spigner C, Allen MD. Ethnic differences in willingness among high school students to donate organs. J Adolesc Health 2006; 39:266-74.13. Chen JX, Zhang TM, Lim FL, Wu HC, Lei TF, Yeong PK, et al. Current knowledge and attitudes about organ donation and transplantation among Chinese university students. Transplant Proc. 2006; 38:2761-5.