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In order to prevent limitation of organ supply, it is needed to raise an awareness about organ donation in public. It is also crucial that health care personnel have knowledge about this subject at the desired level. This research aimed at determining knowledge and attitudes about organ donation of health care personnel working in Tertiary hospital, in which organ transplant center is available.This prospective, cross-sectional research was conducted between 10th January to 01st March 2017 in Gulhane Training and Research Hospital in Ankara, Turkey. A survey consisting of 20 questions about sociodemographic characteristic and organ donation was used. 1200 survey were distributed, 1012 of them were analyzed (84,3%).Age interval of the participants was 18‐63 years. Of 1012 participants, 241 were physicians (23,8%), 202 were nurse (20,0%), 220 were health technicians (21,7%) and 349 were non-clinical staffs (34,5%). It was stated, of participants, 59,6% had enough information about organ donation, 42,6% used media and internet as an information source. It was found that 52,8% of health care personnel were volunteer for organ donation, however, 16,7% of them had organ donation card. Religious-traditional factors and personal preferences are one of the most important reasons of not to donate organ. It was found, of the participants, 44,8% would agree to donate their relatives’ organ in case of brain death, 94,5% would agree to be transplanted organ in case of organ failure of them or their relatives. Additionally, 70,8% stated they believed brain death was real death. Occupation and monthly income of sociodemographic characteristic were found to have statistical effect on voluntariness for organ donation (p<0,05).More than half of health personnel was volunteer for organ donation and have enough information about this subject. Physicians are more favorable about organ donation than other health care personnel.1. Dongmei Hu, Hai Huang. Knowledge, Attitudes, and Willingness Toward Organ Donation Among Health Professionals in China. Transplantation. 2015; 99:1379–1385.2. Antonio Ri´os, Ana Lo´pez-Navas, Marco Antonio Ayala-Garci´a, Mari´a Jose´ Sebastia´n, Anselmo Abdo-Cuza, Jeannina Ala´n, Laura Marti´nez-Alarco´n, Ector Jaime Rami´rez, Gerardo Mun˜oz, Juliette Sua´rez-Lo´pez,Roberto Castellanos,Ricardo Rami´rez,Beatriz Gonza´lez,Miguel Angel Marti´nez,Ernesto Di´az,Pablo Rami´rez, Pascual Parrilla. CIRUGI´A ESPAN˜OLA. 2014; 92:393–403.3. Luis Eduardo Hernández-Ibarra, Francisco J Mercado-Martínez, Anabel Martínez-Castañeda, Organ donation and transplantation in Mexico. A transplantation health professionals’ perspective. salud pública de méxico. 2017; 59: 53–58.4. Akgun HS, Bilgin N, Tokalak I, Kut A, Haberal M. Organ Donation: A Cross-Sectional Survey of the Knowledge and Personal Views of Turkish Health Care Professionals. Transplantation Proceedings. 2003; 35: 1273–1275.5. Alsaied O, Bener A, Al-Mosalamani Y, Nour B. Knowledge and Attitudes of Health Care Professionals Toward Organ Donation and Transplantation. Saudi J Kidney Dis Transpl. 2012; 23: 1304–1310.6. Lomeroa MM, Raserob MJ, Fuentesb L, Jaumea M. Knowledge and Attitude of Health Personnel at the Garraf Health Consortium Regarding Donation and Transplantation. Transplantation Proceedings. 2015; 47: 2318–2321.