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We have firstly demonstrated that regulatory T cell (Treg)-enriched cell therapy efficiently promotes an operational tolerance in 7 of 10 cases of living donor liver transplantation (LT). To identify the tolerant immune status and immunological characteristics of the patients following a cell therapy, we evaluated the immune profiles for a total of 5 years including 3.5 years of completely off IS.PBMCs were collected at pre- and post-transplant periods (7, 14 days, 1, 3, 6 months and annually) from the patients including 7 patients who successfully weaned off IS (off IS group) and 3 patients who were unable to be completely off IS due to a rejection (on IS group). Immunological assays including lymphocyte subset analysis (FCM) and allo-immune responses (IFNg ELISPOT and MLR) were performed.The dynamic immune profile showed that the anti-donor reactivity was occasionally observed without histologic evidence of rejection in a tolerant case. Meanwhile IFNg ELISpot and cell proliferation assays displayed a donor-specific hyporesponsiveness at long-term; 5 (2-6) years post-LDLT in all patients although the slightly higher frequencies of anti-donor CD4+ T cell reactivity were found in on IS group. Also an increase in the percentage of CD4+Foxp3+ Tregs during early periods was observed in off IS group (n=7, d7, d14 and d28 post-LT vs. pre-LT, p<0.05, paired T test). Interestingly such phenomenon was found at long-term post-LT (15.9% in off IS vs. 6.47% in on IS). Furthermore, CD4+ lymphocytes in the peripheral blood obtained from the patients in on IS group (n=3) at pre- and early duration post-LT, were expressed CD45RA and CD62L (so-called naïve subset, *p<0.05 vs. off IS, Fig. A). In contrast, a significant higher percentage of CD4+ memory T cell, namely central memory (CD45RA-CD62L+, Tcm) population was found in off IS patients (n=7, *p<0.05 vs. on IS, Fig. B).The dynamic profile of immune status after an infusion of Treg-enriched cell products for a long-term revealed that the lymphocytes subset such as CD4+ Treg and Tcm during peri-transplant periods may be associated with an induction of immune tolerance by a cell based treatment.A grant in aid from the Japanese Ministry of Health, Labour and Welfare (22130701).