CD8 T Effector Memory T Cells as Predictive Biomarker of Severe Acute Rejection in Lung Transplantation

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Abstract

Material and Methods

76 patients with end-stage lung disease were recruited from 2010 in our institution and prospectively followed after LT. Flow cytometry and monoclonal antibodies to identify CD8 T cell subsets in peripheral blood were used. Protocol biopsy at 21 day post transplantation was performed to identify acute rejection.

Results and Discussion

A total of 41 (54%) of LT recipients had acute rejection and 9 of them were severe (grade A3 or A4). Before transplant, the patients with severe acute rejection had significantly higher CD8 TEM/mm3 than no AR group (median 328.5; IQR: 187.2-507.6) vs (median 70.2; IQR: 49.3-123.8); p=0.027, whereas the levels of CD8 TEM in mild AR group are reduced compared with severe AR (median 95.4; IQR: 45.8 – 189.8); p=0.0748. The CD8 TEM levels pre-LT are increased in those with subsequent severe AR.

Conclusion

Monitoring the levels of CD8 TEM prior lung transplantation could identify patients at risk of suffering severe acute rejection. This biomarker should be validated in larger multicentre studies before be used in clinic.

Reference

San Segundo, D et al. PLoS One. 2013 Nov 13;8(11):e80601

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