Multivisceral Xenotransplantation: The Effect of Different Dpecies Combination

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Abstract

Method

Multivisceral grafts (esophagus, stomach, small intestine, colon, liver, pancreas, spleen and kidneys) were recovery and implanted heterotopically in dog-to-pig (n = 5); pig-to-dog (n = 5); rabbit-to-pig (n = 15) combinations. Multivisceral allotransplantation [pig-to-pig (n=5), dog-to-dog (n=4) and rabbit-to-rabbit (n=5)] composed the control group. Three hours after reperfusion, we collected samples from the graft for histopathology.

Results

HR was visually noted in xenografts around 15 minutes after reperfusion. The autopsy revealed predominance of mild and moderate HR in esophagus and liver in all combination and stomach in pig-to-dog and dog-to-pig combination and severe HR was predominant in stomach, small intestine, colon, pancreas, spleen and kidneys. The occurrence of HR was absent in allografts. IgG fixation was strong in xenografts and absent in allografts.

Conclusion

The three different models of this experiment are relevant to study HR and achieved similar evolution. HR was less aggressive in esophagus, stomach and liver, while it was severe in small intestine, colon, pancreas, spleen and kidneys. allografts reveled normal appearance or mild reperfusion injury in all organs.

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