PREPARATION OF ANTISERA WITH SPECIFICITY FOR HUMAN T LYMPHOCYTES

    loading  Checking for direct PDF access through Ovid

Abstract

SUMMARY

Antisera were raised in rabbits and sheep against human or monkey thymocyte or lymphocyte antigens. The unabsorbed antisera reacted equally strongly with T and B lymphoblasts. After partial absorption with B lymphoblasts antisera were specific for T lymphoblasts when tested by cytotoxicity, but still reacted with B lymphoblasts in indirect immunofluorescence. Extensive absorption with reusable columns made from B cell antigens produced sera which reacted selectively with T lymphoblasts and T leukaemic cells and were able to differentiate T and B cells in human peripheral blood.

    loading  Loading Related Articles