Effect of thrombopoietin alone and a combination of cytochalasin B and ethylene glycol bis(β-aminoethyl ether) N,N′-tetraacetic acid-AM on the survival and function of autologous baboon platelets stored at 4°C for as long as 5 days

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Abstract

BACKGROUND

PLTs stored at 22°C have the potential for bacterial contamination, a problem that could be reduced by 4°C storage. Nevertheless, PLTs stored at 4°C exhibit a significantly reduced life span. This study was performed to determine whether treatment of PLTs with thrombopoietin or cytochalasin B plus ethylene glycol bis(β-aminoethyl ether) N,N′-tetraacetic acid (EGTA)-AM could prevent exponential loss of PLTs stored at 4°C.

STUDY DESIGN AND METHODS

Autologous baboon PLTs were stored at 22 or 4°C. The 4°C stored PLTs were treated with 1.5 ng per mL thrombopoietin, with 1 μmol per L cytochalasin B, and 80 μmol per L EGTA-AM (cyto-EGTA) or not treated and labeled with 111In-oxine to study their in vivo recovery and life span. PLT function was assessed by correction of an aspirin-induced prolonged bleeding time. Aggregation responses and morphology were also assessed.

RESULTS

PLTs stored at 22°C had normal in vivo recovery and linear survival. PLTs stored at 4°C, whether or not they were treated with thrombopoietin, had normal recovery and exponential survival. Aggregation of cyto-EGTA-treated PLTs was similar for PLTs stored at 4°C and fresh PLTs, but decreased in PLTs stored at 22°C for 5 days. The addition of cyto-EGTA to PLTs before 4°C storage inhibited morphologic changes that occurred in PLTs stored at 22°C and cold-induced PLT clumping, but did not prevent exponential disappearance of the PLTs.

CONCLUSION

Addition of thrombopoietin or cyto-chalasin B and EGTA-AM to PLTs before 4°C storage did not prevent exponential loss of PLTs.

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