In vitro and in vivo evaluation of LEUKOSEP HRC-600-C leukoreduction filtration system for red cells

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Documentation of the benefits of leukoreduction has led to the increased use of this technique and the need for development of efficient and effective techniques for its accomplishment. This study investigated the in vitro properties and in vivo autologous radiolabeled recovery of leukoreduced red cells (RBCs) produced through a leukoreduction filtration system for RBCs (LEUKOSEP HRC-600-C, Hemerus Medical).


Normal subjects donated 36 units of RBCs that were leukoreduced on Days 0, 3, or 5 through a “hands-off” technique. Biochemical studies were performed before and after filtration and at the end of 42 days of storage. Units leukoreduced on Days 0 or 5 were held until Day 42 and used for autologous radiolabeled return to determine recovery with 51Cr single-label radiolabeling techniques.


Leukoreduction filtration was accomplished in 16.3 ± 2 minutes on Day 0 at room temperature or 27 to 30 minutes on Days 3 or 5 after refrigeration. Leukoreduction efficiency was 4.6 ± 0.6 log with a median residual white blood cell (WBC) content of fewer than 3.3 × 104 WBCs per unit. RBC recovery was 90 ± 2 percent. Hemolysis was 0.34 ± 0.16 percent at the end of 42 days of storage. The in vivo recovery of radiolabeled RBCs 24 hours after autologous return was 80.6 ± 4.5 percent for RBC units leukoreduced on Days 0 and 5 combined.


The LEUKOSEP HRC-600-C WBC reduction filtration system produced leukoreduced RBCs efficiently and effectively with acceptable poststorage biochemical measures and posttransfusion recovery after 42 days of storage.

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