Effects of granulocyte–colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors

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Abstract

BACKGROUND:

Reports of Monosomy 7 in patients receiving granulocyte–colony-stimulating factor (G-CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G-CSF produces Monosomy 7 aneuploidy in healthy donors.

STUDY DESIGN AND METHODS:

We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G-CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G-CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.

RESULTS:

We found no abnormalities in Chromosomes 7 and 8 in G-CSF–mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G-CSF– and Dex-treated donors.

CONCLUSION:

G-CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.

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