Mur (MNS 10) screening with a novel loop-mediated isothermal amplification assay in Zhongshan, China

    loading  Checking for direct PDF access through Ovid

Abstract

Background:

The distribution of the Mur blood group antigen is 5–7% in the south of China, and a much higher prevalence is observed in some areas of the region. Anti-Mur can cause hemolytic disease of the newborn and severe transfusion reactions.

Objectives:

Genetic testing is more ideal than conventional serological tests because antibodies for detection are usually not available.

Methods:

In this study, a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Mur blood group antigen was established.

Results:

Fifteen of 275 (5·5%) samples were confirmed by LAMP as Mur antigen positive. All the Mur antigen-positive samples were GP.Mur subtype which was confirmed with sequencing.

Conclusion:

The LAMP method has identical results with conventional serology method but more suitable for large-scale screening.

Related Topics

    loading  Loading Related Articles