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A novel ultrasonic vibration-assisted (UV-A) electrosurgical electrode was firstly proposed.The adhesion force with UV-A was decreased 60–75% without affected by load.The mass of adhesion tissue was decreased by 80% with UV-A.The underlying mechanism with UV-A for soft tissue anti-adhesion was revealed.The electrosurgical electrode currently stands out as one of the most commonly used tools in minimally invasive surgery. In order to facilitate tissue cutting and accelerate wound healing, tissue adhesion to the electrosurgical electrode is considered as an extremely urgent problem to be solved. In this paper, a novel ultrasonic vibration assisted (UV-A) electrosurgical electrode is firstly proposed to overcome the problem of tissue sticking. The anti-adhesion effects were evaluated by measuring the adhesion force and the weight of tissue adhesion using the electrosurgical electrode with and without UV-A comparatively. Experimental results show that the average adhesion force and the tissue adhesion mass with UV-A were decreased by approximately 60% and 70% respectively, accompanied by smaller thermal injury area compared with that without UV-A. Moreover, the underlying mechanism of anti-adhesion effect with UV-A was revealed by investigating the influence of ultrasonic vibration on electric current, tissue removal and spark discharge. This research suggests that UV-A is a promising and practical method for improving the anti-adhesion performance of electrosurgical electrode.