To determine changes in urine neutrophil gelatinase-associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid-based fluids.Study design
Prospective experimental study.Animals
Seven healthy adult entire male Greyhound dogs.Methods
During isoflurane anaesthesia, approximately 50 mL kg−1 of blood was removed to maintain mean arterial pressure (MAP) ≤40 mmHg for 1 hour followed by gelatin-based colloid administration to maintain MAP ≥60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2–T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated-measures one-way anova. Data are presented as means (95% confidence interval).Results
Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0–30.6) ng mL−1 at T0 to 122.0 (64.1–180.0) ng mL−1 by T3. Compared with T0, uNGAL was significantly higher at T3 (p = 0.016) and was increased 24-fold.Conclusions and clinical relevance
Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage-induced hypotension and colloid-mediated reperfusion.