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Black-Fronted Piping-Guan (Aburria jacutinga) is considered one of the most threatened bird species on the American continent. Ex situ conservation efforts have been developed in Brazil, and monitoring the health status of these populations is essential to ensure reproductive success in captivity. Reference intervals (RI) for biochemistry analytes from a larger population of Black-Fronted Piping-Guan are unavailable.This study was aimed at establishing RI for biochemical analytes of captive Black-Fronted Piping-Guan for future improved health assessments in these animals.Blood samples were collected from 32 adult Black-Fronted Piping-Guans for serum and plasma separation. Liver and kidney function markers, and concentration of protein and lipids were measured. Reference intervals were determined using an Excel program with Reference Value Adviser (version 2.0).The RI of the analytes were: AST 20.7–188.0 U/L; CK 713.7–4741.4 U/L; LDH 360.7–1173.7 U/L; γ Glutamyl Transferase 1.1–5.4 U/L; total plasma protein 3.5–6.2 g/dL; total serum protein 1.7–4.7 g/dL; uric acid 0.9–19.2 mg/dL; urea 7.0–25.3 mg/dL; cholesterol 71.8–232.2 mg/dL; triglycerides 25.6–183.4 mg/dL.The RI are similar to the ones published for other avian species. Only CK and uric acid values were in discordance with the usually reported avian ranges. This could be due to different methods of capture and physical restraint. Alternatively, some differences could be species-specific. In general, physical restraint should be limited to a minimum and without stress to prevent capture myopathy and death.