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Frequencies of blood types A, B, and AB in domestic cats vary geographically and among breeds and have not been reported in China.The purpose of this study was to survey the frequency of blood types in domestic cats in the Beijing area.A total of 262 cats from the city of Beijing were blood-typed using a standard tube agglutination assay. All cats were nonpedigree domestic shorthaired and longhaired cats; purebred cats were excluded. Serum obtained from type-B cats and a lectin (Triticum vulgaris) solution served as anti-A and anti-B reagents, respectively. The presence of alloantibodies was also determined in some cats.The frequency of blood types was 88.2% type A, 11.4% type B, and 0.4% type AB. The tube assay resulted in 3+ to 4+ agglutination reactions with either the anti-A or anti-B reagents. The 1 type AB sample showed 3+ agglutination with both anti-A and anti-B reagents; the plasma of that sample did not react with either type-A or type-B RBCs. Tested type-B cats had strong anti-A antibodies.The frequency of blood type B in the Beijing area was relatively high and similar to that reported for other Asian countries and Australia. Blood-typing is recommended to match donors and recipients before transfusion therapy and planned matings to avoid hemolytic transfusion and neonatal isoerythrolysis reactions, respectively, due to blood-type incompatibility.