Detection of Rabies Virus Genes by In-Situ Polymerase Chain Reaction


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Abstract

Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.

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