Staphylococcus saprophyticussurface-associated protein (Ssp) is associated with lifespan reduction inCaenorhabditis elegans

    loading  Checking for direct PDF access through Ovid


Staphylococcal lipases have been proposed as pathogenicity factors. InStaphylococcus saprophyticusthe surface-associated protein (Ssp) has been previously characterized as a cell wall-associated true lipase. aS. saprophyticusΔssp::ermB mutant has been described as less virulent in an in vivo model of urinary tract infection compared with its wild-type. This is the first report showing thatS. saprophyticusinduced a lifespan reduction inCaenorhabditis eleganssimilar to that ofS. aureusRN4220. In twoS. saprophyticusΔssp::ermB mutants lifespan reduction inC. eleganswas partly abolished.In order to attribute virulence to the lipase activity itself and distinguish this phenomenon from the presence of the Ssp-protein, the conserved active site of the lipase was modified by site-directed ligase-independent mutagenesis and lipase activity-deficient mutants were constructed. These results indicate that the Ssp is associated with pathogenicity inC. elegansand one could speculate that the lipase activity itself is responsible for this virulence.

    loading  Loading Related Articles