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In a short period of time, a large number of adventitious shoots were regenerated from longitudinal sections of in vitro-germinated seedlings of the endangered Mexican cactus, Turbinicarpus laui. The induction medium consisted of Murashige and Skoog salts, supplemented with 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA) in a wide range of combinations. The most effective concentrations of growth regulators for shoots initiation were 8.8-13.32 μM BA with 0-2.68 μM NAA. After 3-4 mo., individualized shoots were rooted on half-strength Murashige and Skoog medium and then transferred to soil to acclimatize under greenhouse conditions. In vitro strategies play a key role in the conservation and propagation of this commercially important endangered species of cactus.