A new transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 antibody

    loading  Checking for direct PDF access through Ovid

Abstract

Background and Objectives

CD36 antibody (Ab) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non-haemolytic transfusion reactions. However, there is no gold-standard test for CD36 Ab.

Materials and Methods

We developed a transfectant panel cell line-based MoAb-independent antigen capture assay system for detection of CD36 Ab and compared it with the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) system in terms of sensitivity and specificity.

Results

Our new system was characterized by (1) gene-transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD36 MoAbs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system.

Conclusions

The present results indicate that our new system permits highly sensitive and specific detection of CD36 Ab.

Related Topics

    loading  Loading Related Articles