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In this paper, we present a Web-based information system for pollution monitoring and analysis of Lake Ohrid. Our work is related to the ecological state of Lake Ohrid, which represents a rare natural ecosystem inhabited by many endemic and relict species. Aim of the study is to present the possibilities offered by the Web application, such as fast information access, support of distributed collaboration by information sharing instead of exchange, acquisition and analysis of pollutant data, database processing based on lake models, as well as GIS analysis. In this way, the data and the analysis are performed in one place and they are available to any user having only a Web browser.