Effect of agitation rate on the growth and production of xylanase free of cellulase by Thermomyces lanuginosus MH4 in bioreactor

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Abstract

Cellulase-free xylanase production by T. lanuginosus MH4 was investigated in a 3-litre stirred tank bioreactor under different agitation rates and an aeration rate of 1 v/v/m. The cultivation time in the bioreactor was reduced significantly over that in shake culture conditions. A xylanase productivity of 0.1 mkat 1−1 h−1 was achieved on xylan in the bioreactor. This was nearly double to that obtained in shake culture. The agitation rates influenced both growth and enzyme secretion in the bioreactor. The highest level of biomass concentration and activities of both xylanase and β-xylosidase were obtained at 150 rev min−1

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