Tannase production by Aspergillusfumigatus MA under solid-state fermentation

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Abstract

A tannase yielding fungal culture identified as Aspergillusfumigatus MA was isolated from the effluent collected from a local small scale tannery. The fungal culture produced high yields of extracellular tannase under solid-state fermentation (SSF) using different agro forest residues such as Amla leaves (Phyllanthusemblica), Ber leaves (Zyzyphusmauritiana), Jamun leaves (Syzygiumcumini), Jamoa leaves (Syzygium sp.) and Keekar leaves (Acacianilotica). Among different substrates used, Jamun leaves yielded maximal extra-cellular production of tannase. Various parameters were studied to optimize the extracellular yield of tannase under SSF. The maximum yield of 174.32 U g−1 was obtained at 25°C after 96 h of incubation at pH 5.0. The tap water was used as a moistening agent. A substrate to tap water ratio of 1:1 was found to best for tannase production. Supplementation of the medium with ammonium sulfate as nitrogen source had enhanced tannase production whereas glucose had decreased the enzyme production. This is the first report on production of tannase by Aspergillusfumigatus MA, giving a much higher yield of enzyme under SSF with Jamun leaves as the substrate.

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