Purification of polyhydroxybutyrate produced by Burkholderia cepacia IPT64 through a chemical and enzymatic route

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Abstract

This work aimed at the study of purification of biopolymers produced by Burkholderia cepacia IPT64, through a chemical attack like an adjuvant procedure through chemical and enzymatic route. Enzymatic lysis using protease was run on an enzyme/cell ratio of 0.02. Chemical attacks were performed as pre or post-treatment for enzymatic attacks, using sodium dodecyl sulphate or hydrogen peroxide. The same chemicals and procedures were used alone as a control. Fenton's reagent was also tested as a chemical treatment. Using only one of the chemicals H2O2, Fenton's reagent or SDS, the purity increase achieved values of 14%, 16% or 23%, respectively. When H2O2 or SDS were used as pre-treatment for the enzymatic attack, the results of purity increase achieved values of 58% for H2O2/cell ratio between 0.60 and 1.20, and 57% when SDS/cell ratio at 0.56 was used. In the case when H2O2 or SDS were used as post-treatment for the enzymatic attack, results of purity increase achieved 60% when the H2O2was used at H2O2/cell ratio ranging between 0.30 and 0.60 and 71% when SDS was used at a ratio SDS/cell of 0.56.

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