Xenotransplant News
Xenotransplantation Literature Update May–June, 2005
Blood group ABO system – an update
Engineering the surface of red cells with synthetic glycolipids (KODET™ CAE) to create ABO analytical sensitivity controls and xeno-modified cells
ABO antigen expression on the graft endothelium long-term after ABO-compatible but non-identical heart transplantation
A survey on assay for anti-A/B antibody by Japanese Society for ABO-incompatible transplantation
Enzymatic removal of blood group A/B antigens
Structural characterization of blood group A glycolipids in blood group A1 liver tissue in situ perfused with O blood
Present status of ABO-incompatible kidney transplantation in Japan
Anti-CD20 and plasmapheresis in ABO-incompatible renal transplantation
The use of rituximab and antigen-specific immunoadsorption in ABO-incompatible kidney transplantations without splenectomy
“Pin-point” targeted immunosuppression anti-CD20/MMF desensitization combined with anti-CD25 in successful ABO-incompatible kidney transplantation without splenectomy
The Göteborg/Birmingham experience of ABO-incompatible renal transplantation using carbohydrate based immunoadsorption and anti-CD20 mAbs
Clinical experience with ABO-incompatible heart transplantation
Successful ABO-incompatible heart transplantation in two infants in Germany
Heart transplantation across the antibodies against HLA and ABO - Post-transplant follow-up
Dramatic improvement in the outcome after ABO-incompatible liver transplantation from living donor by hepatic arterial infusion therapy
The usage of rituximab for adult living donor liver transplantation across ABO blood type barrier
Adult ABO-incompatible liver transplantation. A single center experience
ABO-incompatible kidney transplantation using antigen specific immunoadsorption and rituximab
ABO-incompatible kidney transplantation of an eight year-old girl with donor/recipient-constellation A1B/B
Splenectomy and antibody depletion may not be needed in B-incompatible kidney transplantation
Post-transplant plasmapheresis is not necessary for ABO-incompatible living donor kidney transplantation
ABO-incompatible kidney transplantation with anti-CD20 MAbs, IVIG, and plasmapheresis
First two successful ABO-incompatible living renal transplantations using splenectomy, rituximab, plasmapheresis and IvIg as a pre-conditioning regimen – A single center experience in the Balkans
ABO-incompatibility in allogeneic hematopoietic stem cell transplantation
No evidence for endothelial cell chimerism after ABO-incompatible allogeneic hematopoietic stem cell transplantation
Rituximab and the suppression of human immunity
Human ABO-incompatible organ transplantation as a clinical surrogate for discordant xenotransplantation
Xenotransplantation using GalT-KO pigs
GalT-KO Organs-where do we go from here?
Hepatocyte xenotransplantation
Preclinical islet xenotransplantation
Pathophysiology of the xenogenic cell-human blood interphase.
Self and non-self recognition by C-type lectins on dendritic cells
The molecular specificity of natural killer cells
The role of alpha-gal in the interaction of innate immune cells and porcine endothelium
Antibody mediated xenograft rejection and protective mechanisms
Induction, maintenance and breaking of xenotolerance in the non-T cell compartment.
Mixed chimerism and tolerization of human T cells to pig xenoantigens
Regulatory issues-an update
Xenosis and xenotransplantation
PERV genetics
Endothelial cytoprotection in xenotransplantation
Dysregulated coagulation in hyperacute lung rejection
Xenogeneic coagulation cascade incompatibilities
Governing hybrids-regulatory species boundary change in xenotransplantation and tissue engineering
Xenotransplantation-ethics and regulation
The xenotransplantation narratives of nine type 1 diabetic patients with renal failure
Pigs, profits and problems. Research ethics in the biotech era
Xenotransplantation, chimerism, and personal identity debates
Immunobiology of the brain as a transplantation site: limitations of the immune privilege
Transplantation for neurodegenerative disorders of the CNS is there a prefential advantage in using xenogeneic tissue?
Transgenic neural expression of huCTAL4-Ig in pigs for neural grafting
Further characterization of the anti-carbohydrate antibody response in patients transplanted with fetal pig islet-like cell clusters using glycan arrays
GALα1,3GAL expression on porcine endothelial cells is crucial to complement-mediated lysis but not NK cell-mediated cytolysis
Gal-alpha(1,3)Gal synthesized by iGb3 synthase elicits antibodies in mice with deletions in the alpha-1,3galactosyltransferase gene
Immunogenicity of cloned pig cells expressing only 1% of alpha Gal antigens
Carbohydrate phenotyping of endothelial cell glycoproteins from alpha1,3galactosyl-transferase gene-knockout and wild type pigs
Human natural antibodies directed against endothelial cells derived from Gal-alpha(1,3)Gal knock-out pigs
Endothelial cell activation in accommodated CTLA4-Ig-treated hamster heart xenografts after cyclosporin A withdrawal
Stem cell engraftment in a heterotopic cardiac hamster-to-rat xenotransplantation model
The double-expression of human decoy Fas antigen and membrane-bound human FasL in xenograft cells can inhibit human CD8 + cytotoxic T lymphocytes-mediated cytotoxicity
Transgenic expression of antioxidant enzymes protects mouse islets from oxidative injury mediated by pro-inflammatory cytokines
Production of swine lacking the gene for [ALPHA] 1,3 galactosyltransferase and expressing the gene for human complement regulatory proteins
Effect of various forms of DAF(CD55) on complementmediated xenogeneic cell lysis
Beyond Gal-knockout
GalT K/O pig lungs are protected from hyperacute rejection by human blood
Anti-non-Gal antibodies play a major role in the development of acute humoral xenograft rejection of hDAF transgenic porcine kidneys in baboons receiving anti-Gal antibody depletion therapy
Thrombotic microangiopathy due to humoral rejection in cardiac xenografts from alpha1,3-galactosyltransferase gene-knockout (GalT-KO) pigs in baboons
Acute and chronic rejection in cardiac xenografts from α1,3-galactosyltransferase gene-knockout (GalT-KO) pigs in baboons
Orthotopic pig to primate cardiac xenotransplantation
Cardiac xenotransplantation
Xenograft rejection without an anti-gal antibody gal antigen interaction
Prolonged function of porcine islet tissue using chimeric donor pigs without chronic immune suppression
Low molecular weight dextran sulfate unlike heparin abrogates the instant blood-mediated inflammatory reaction in pig-to-monkey pre-clinical islet xenotransplantation
Clinical trial with pig hepatocyte-AMC-bioartificial liver in patient with acute liver failure
Targeted deletion of Fgl2/Fibroleukin in the donor modulates the immunologic response and acute vascular rejection in cardiac xenografts
Thrombin is required for inflammatory cell recruitment and graft infiltration during acute humoral xenograft rejection
Characterization of pig thrombomodulin and its interaction with the human protein C anticoagulant pathway
Transgenic CD39 expression protects against renal ischemia reperfusion injury
Role of Growth arrest specific gene 6 in immune and non-immune aspects of vascular lesions occurring during delayed xenograft rejection
The role of coagulation cascade activation in early failure of transgenic pig hearts in baboons
Recombinant human activated Protein C (rh APC) in pig-to-primate xenotransplantation
Natural killer cells are required for rapidly induced xeno-antibody production by marginal zone B cells
Long-term survival of hamster heart xenografts in IL-7 knock-out mice
Development of xenotolerance in (CD4 + or CD8 +) T-dependent B lymphocytes after their exposure to xenoantigens in the absence of T lymphocytes
B-cell extrinsic complement receptor 1/2 is required for natural antibody production and for tolerization of B-1 cells producing anti-aGAL via mixed chimerism induction
Allosensitized humans are at no greater risk of humoral rejection of GT-KO pig organs than other humans
Absence of correlation between the degree of anti-human panel reactive antibodies and reactivity to cells from GalT knock-out swine in patients awaiting cadaveric renal transplants
Incidence and cytotoxicity of antibodies in cynomolgus monkeys, baboons and humans directed to non-gal antigens
Further characterization of tilapia islet rejection in mice
No effect of indirect antigen presentation impairment on the capability of CD8 T cells to mediate acute tilapia islet rejection in mice
Prolongation of tilapia islet xenograft survival using everolimus (RAD) and FTY720
Six-month immune protection of porcine islets transplantaed in non-diabetic cynomolgus monkeys without immunosuppression
Long-term gene expression and metabolic control exerted by lentivirus-transduced pancreatic islets
Tolerance induction to xenogeneic islets by anti-CD154 mAb and rapamycin is based on anergy and regulation
Cobalt protoporphyrin treatment of neonatal porcine islets reduces caspase-3,-7 activation but cannot protect islets from TNF-αlpha mediated damage
Immune modulation of microencapsulated neonatal porcine islets by in vivo maturation in immune-deficient mice
Passive transfer of peritoneal CD4+ T cells, but not CD8+ T cells, from NOD mice rejecting encapsulated porcine islets causes rapid destruction of encapsulated porcine islets in NOD Scid mice
PERV encodes major xenoantigens for the rejection of porcine cellular xenografts
Identification of signaling pathways involved in macrophage recruitment to porcine xenograft
Chemokine and toll-like receptor signaling in macrophage mediated islet xenograft rejection
Defects in the CD4 + CD25 + (regulatory) T-cell compartment in mice developing a multiorgan auto-immune syndrome after xenogeneic thymus transplantation
Description of a novel method of xenothymus transplantation without development of a multi-organ auto-immune syndrome
Evaluation of in vivo T-cell responses to pig to primate cardiac xenotransplantation
A subset of human xenoreactive CD8 + T cells express CD94/NKG2A and their cytotoxicity can be inhibited by an HLA-E single chain trimer
Tissue accommodation induced in chimeric donor pigs
Porcine endogenous retrovirus and its transmission characteristics
Development of strategies to prevent transmission of porcine endogenous retroviruses (PERVs) following xenotransplantation
In vitro infection of human fibroblasts with porcine cytomegalovirus
Genomic characterization of the porcine endogenous retrovirus gamma 2 family
Assessment of PERV infection in patients treated with Academic Medical Center bio artificial liver
Effects of carbon monoxide-releasing molecule (CORM-3) on porcine endothelial cells and primate PBMC
Anti-inflammatory responses and tolerability following the in vivo administration of a carbon monoxide-releasing molecule in primates
A novel in vitro model using whole blood to study endothelial cell protection and complement inhibition in xenotransplantation
Porcine endothelial cells transduced with adenoIL-4 are protected from injury caused by human complement through Akt activation
Porcine iliac arteries transduced in vivo with adenoIL-4 are protected from acute injury caused by ex vivo perfusion with human blood
Cloning and a trial to protect apoptosis by pig anti apoptotic inhibitors
Recruitment of human leukocytes to porcine endothelial cells expressing or lacking Gal-alpha (1,3) Gal
Molecular studies of human NK cell recognition of xenogeneic carbohydrate epitopes
aGal antigen modulates the expression of pro-inflammatory genes in hyperacute lung rejection
Human NK cytotoxicity against porcine endothelial cells is triggered by NKp44 and NKG2D
Molecular cloning of potential porcine endothelial cell ligands for the human NK cell activating receptor, NKG2D
Pig donor size affects porcine islet isolation yield and islet survival
Encapsulated human islet therapy for diabetes mellitus
Rat kidney subcapsular space is a more appropriate site than peritoneum or subcutaneous space to transplant pig encapsulated islets
The survival of adult and neonatal porcine islets from N-acetylglucosaminyl-transferase-III (GNT-III) transgenic pigs in cynomolgous monkeys
Costimulation blockade enables long-term survival of porcine islet xenograft in C57BL/6 mice
Short-term CTLA4-Ig and rapamycin therapy significantly prolongs survival of islet xenografts
Prolonged survival of microencapsulated neonatal porcine islets in mice treated with a combination of anti-cd154 and anti-lfa-1 monoclonal antibodies
Streptozotocin administration in young tethered monkeys-early response, dosage, and side effects
Microencapsulation of porcine pancreas cells with sodium cellulose sulfate (NaCS) to create a bioartificial pancreas
Hyperbaric oxygenation and porcine islet isolation
Co-transplantation of GLP-1 secreting tilapia islets does not enhance beta-cell differentiation in neonatal porcine islet cell clusters
Early recruitment of dendritic cells in the rat brain parenchyma after intrastriatal transplantation of porcine ventral mesencephalic neurons
Minocycline delays the rejection of an intracerebral cell xenograft
RNAi-mediated gene silencing of porcine CD86 prevents costimulation of human anti-porcine T cell response
Adeno-associated virus serotype 2 efficiently transduces primary porcine endothelial cells
Significant prevention of human CD8 + cytotoxic T lymphocytes (CTL)-mediated cytotoxicity toward swine endothelial cells by overexpression of human decoy Fas antigen
Use of lentiviral vectors for combination of transgenic strategies to facilitate pig-to-primate xenotransplantation
Adhesion and function of human hepatocytes on biomodified collagen-poly (lactic acid) porous sponge scaffold
Successful production of cloned pigs with high expression of human DAF by selecting donor cells for nuclear transfer using cell sorter
Generation of hHO-1/GFP transgenic animals for xenotransplantation of tissues and cells
Human neutrophils gene expression profiling following xenogeneic encounter with porcine aortic endothelial cells
Macrophage and NK cell-dependent rejection in the mouse heart-to-rat model
Higher-level expression of UL18 by tail-less mutation conferred more effective control of NK-mediated swine endothelial cell cytotoxicity
Interspecies difference in thiopurine S-methyltransferase activity between human and porcine
Local effector mechanisms of corneal xenograft rejection
Morphological and functional characterization of porcine dendritic cells
Arginase activity regulates nitric oxide production in rejected skin xenografts
Porcine CD34 + HSC isolation for chimera development
Optimal developmental stage of Sertoli cells for maximum immune protection
Selective depletion of human activated T cell by anti-CTLA-4 human scFv-perforin immunotoxin
The prompt function of recombinant activated T cell specific membrane active immunotoxin (IT)
Buccal mucosal cell immunohistochemistry-a simple method of determining the ABH phenotype of pigs, monkeys, and baboons
Differential binding of Griffonia simplicifolia 1 isolectin B4 (GS1B4) to alpha-galactose antigen in porcine adrenal gland
Detection of gal alpha 1,3-gal level in pigs using chicken IgY antibody
Biochemical studies of Gal antigens in alpha1,3-galactosyltransferase knockout porcine small intestine and heart tissue
Biochemical studies of Gal antigens in small intestine and pancreas from alpha1,3-galactosyltransferase knock-out miniature swine
Hdaf on pig cells protects against primate sera cytotoxicity mediated by exogenous or endogenous complement, as determined by flow cytometry
Neonatal porcine Sertoli cells inhibit human natural antibody-mediated lysis by preventing formation of the membrane attack complex
Preformed and elicited anti-non-gal antibodies in naive baboons and after exposure to a GT-KO pig heart
Roles of classical and alternative pathways of complement activation in hyperacute rejection of pig-to baboon kidney xenografts identified by specific complement pathway inhibitors
Antigenicity of porcine cornea as xenograft
Jejunal resection reduces oral immunisation with xenoantigens in the mouse-to-rat transplantation model
The presence of Hanganutziu-Deicher (HD) antigens in humans
Reduction of N-glycolylneuraminic acid (Hanganutziu-Deicher) antigens by RNA interference
Human complement-activating IgG3 antibodies are essential for porcine endothelial cell activation in an in vitro xenotransplantation model
DAF prevents acute humoral rejection induced by low titers of anti-αGal antibodies in GalT KO mice
Molecular cloning and expression of a porcine CC chemokine RANTES chemoattractant for human monocytes and NK cells
Diverse effects of cobalt protoporphyrin in combination with immunosuppressive drugs
Characterization and long-term maintenance of primary isolated porcine endothelial cell for xeno-immune reaction study
Inhibitory effect of activated protein C on coagulation and endothelial activation
Discordant renal xenotransplantation
Histological assessment of gastrointestinal lesions in xenotransplanted primates of Macaca fascicularis recipients hDAF porcine kidneys
Analysis of the affinity of haptoglobin of banna minipig inbred line (BMI) capturing human hemoglobin
Analysis of appropriate donor size for procine to human kidney xenotransplantation
Analysis of appropriate procine liver donor size for xenotransplantation
Heme oxygenase-1 expression and TNF-alpha production following administration of cobalt porphyrins in rodents and primates
Effect of temperature on the perfusion of human blood into isolated kidney of hDAF transgenic pigs
The combination of interspecies immunological and metabolical differences might explain differences in susceptibility to ischemia/reperfusion injury and graft survival after prolonged cold ischemia
Porcine and human hepatocyte isolation, cryopreservation, and xenotransplantation
Intact active bone transplantation synergizes with cyclosporine to induce long-term xenograft survival in a rat-to-mouse cardiac transplantation model
Expression of human CD59 and MCP prolongs transgenic mouse heart function in an ex vivo model of xenograft rejection
Depletion of complement system inhibits early epithelial destruction in porcine bronchial xenograft to mouse
Ex vivo removal of primate anti-pig antibodies
Fluorescent microspheres reveal different regional blood flow in non-transgenic, and hdaf pig hearts perfused with human blood and gas914 or tirofiban
Presence of xenoquimerism in pig-to-baboon heart xenotransplantation with persistent depletion of anti-agal antibodies
Streptozotocin-induced diabetes in large animals (pigs/primates)
Copy number of porcine endogenous retrovirus increases during pig inbreeding
Further study of effects on human cells after long-term infection with porcine endogenous retrovirus
Phylogenetic analysis of porcine endogenous retroviruses (PERV) in chinese pigs based on envelope sequences
The variation of host cell tropism of porcine endogenous retroviruses in chinese banna minipig inbred
Extended characterisation of human cell-adapted porcine endogenous retroviruses (PERVs)
Selection of pigs suitable for human xenotransplant donation
Suppression of the PERV transmission to human cells by neuraminidase
Remodeling PERV envelope N-glycans downregulates their transmission to human cells
Control of human tropic replication competent porcine endogenous retroviruses (PERV) in cells and organs for xenotransplantation.
Effect of 3-year course on the attitude on xenotransplantation among Italian university students